Immunohistochemistry (IHC)

The science of immunohistochemistry (IHC) is evolving, as evidenced by the development of increasingly sophisticated automated staining platforms, production of specific antibodies for a wide range of species and the availability of a range of novel biomarkers.

IHC studies optimize staining protocols for specific target expression/profiling in human and animal tissues that can be used for a variety of reasons, such as proliferation/apoptosis studies, immuno-oncology, ocular tissues, tumor profiling and tissue cross reactivity.

Common IHC Applications

  • Characterize immune cells
  • Evaluate the relationship of tumor cells and immune cells
  • Visualize cells that are not visible on standard H&E e.g. microglia
  • Detect and measure cell proliferation
  • Identify which patients are likely to gain benefit from a drug
  • Assess on or off target binding of an antibody drug
  • Determine the specific location of a protein in a tissue sample i.e. in situ proteomics
  • Track human stem cells xenotransplanted into animal models that are used to study their fate
  • Study drug metabolism e.g. insulin and glucagon
  • Visualize a reporter gene product such as Green Fluorescent Protein (GFP)
  • Comply with guidance such as The Society of Toxicologic Pathology (STP)

GLP IHC Features

  • Automated IHC stainers with onboard antigen retrieval capabilities:
    • Leica BOND RX
    • Ventana Discovery XT
    • Ventana Discovery Ultra
    • Ventana Benchmark Ultra
  • High throughput manual methods using ThermoScientific Shandon Sequenza
  • Tissue cross reactivity studies
  • Immunophenotyping by IHC
  • Multiplexing, including dual IHC/ISH
  • Multi-species optimally processed tissue bank
  • Experience dating back to 1988
  • Hundreds of methods previously developed
  • Scientific and technical expertise for novel method development

Human NSCLC Adeno CD3CD68CK Triple Stain
Human NSCLC CD3 CD68 CK
Mouse spleen CD8
Mouse Spleen CD8
Rat brain GFAP
Rat Brain GFAP
Rat Pancreas - Chromogranin A
Rat Pancreas - Chromogranin A

  • Hundreds of standard and uncommon (or customizable) assays
  • Multiplex staining technologies (chromogenic and fluorescent) 
  • Large bank of optimally processed paraffin embedded and frozen tissues
  • Consistent use of major automated IHC platforms, validated to ensure electronic data regulatory compliance (also see SEND)
  • Standard operating procedures among global facilities in Asia, Europe and North American