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Showing results 1025 - 1040 of 91813 for .

Showing results 1025 - 1040 of 91813 for .

Evaluation of a High-Sensitivity GC-MS/MS in the Analysis of Pesticide Residues in Complex Samples: Cost Savings and Other Benefits
ASMS 2017 -- A high-sensitivity GC-MS/MS instrument was evaluated for improved pesticide multiresidue analysis in complex botanical samples. The increased detection sensitivity was used to decrease the GC injection volume, thus reducing introduction of the sample matrix into the GC-MS/MS system. This translated into substantial cost savings due to less frequent maintenance and also significant improvements in analyte peak shapes in highly concentrated botanical extracts, which would...
Development and Validation of a Large Multiresidue LC-MS/MS Method Using On-Line Dilution and Other Features Useful for Routine Analysis
HPLC Symposium 2017 -- The aim of this work was to develop and validate a pesticide multiresidue LC-MS/MS method for the analysis of about 450 analytes eluting in less than 10 min. The MS/MS acquisition method employs triggered multiple reaction monitoring (tMRM), which provides increased confidence in analyte identification through triggered acquisition of additional MRMs when one of the primary MRMs exceeds a set abundance threshold (> 2000 MRMs included in the method). The mobile phase...
Comparative Approaches Between Quantitative-MSI and Quantitative-WBA: Application to Chloroquine Administration in a Long-Evans Male Rat Model
ISSX 2016 -- Mass Spectrometry Imaging (MSI) and Quantitative Whole Body Autoradiography (QWBA) are related techniques to assess the distribution of drug related material. While QWBA is the gold standard for quantification in biological tissues the technique does not allow individual components to be identified. MSI has long been used to provide molecular distribution information in a specific mass range and has recently shown capabilities in quantifying material in biological tissues...
Quantitative Determination of AVI-7100, a Phosphorodiamidate Morpholino Oligomer (PMOplus®), in Human Plasma Using LC-MS/MS
AAPS 2015 - AVI-7100 is a positively charged phosphorodiamidate morpholino oligomer (PMOplus®). AVI-7100 is designed to bind to viral RNA and inhibit the synthesis of the M1 and M2 matrix proteins. Inhibition of the M1 and M2 matrix proteins is intended to disrupt viral replication and slow the spread of the disease to other cells in the body, thereby allowing the adaptive immune system the opportunity to mount a response.
Developing Ultra-Sensitive Bioanalytical Method to Quantify Monomethyl Auristatin E (MMAE) at 1 pg/mL Using Heart-Cut 2D-LC-MS/MS
AAPS 2015 - Monomethyl auristatin E (MMAE) is a synthetic antineoplastic agent. Because of its toxicity, it is typically linked to a monoclonal antibody which directs it to the cancer cells. Because MMAE is covalently bound to the antibody and is intended to be released only at the target cancer cell, the plasma concentrations of free MMAE are normally quite low. Here we report a "heart-cut" 2D-LC-MS/MS method with peak summing for the quantitation of MMAE in monkey plasma with a...
Overcoming Non-Specific Binding During the Quantitation of the RG-012 Active Ingredient and Its Major Active Metabolite in Human Urine Using LC-MS/MS
AAPS-NBC 2016 - RG-012, a microRNA therapeutic being investigated for the treatment of Alport syndrome, is a single-stranded chemically modified oligonucleotide that binds to and inhibits the activity of miR-21, a microRNA associated with kidney stress and renal dysfunction. During method development for the simultaneous analysis of the RG-012 active ingredient (RG456070) and its (RG0005) in human urine using LC-MS/MS, severe non-specific binding of the analytes in urine samples was...
Quantification of Two Synthetic, Double-Stranded, RNAi Duplexes Using Hybridization with HPLC-Fluorescence
AAPS 2016 -- Using a proprietary Dynamic Polyconjugate (DPCTM) technology, ARC-520 RNAi therapeutics, a liver-targeted antiviral drug, was formulated in which the active pharmaceutical ingredient (API) contained a mixture of two synthetic, double-stranded, RNAi duplexes, AD0009 and AD0010. Four Hybridization HPLC-fluorescence methods were developed to quantify the two RNAi duplexes with great reproducibility, selectivity and sensitivity in human plasma from 1-100 and from 1,000-100,000 ng/mL.
Simultaneous Quantitation of MicroRNA Therapeutic RG-012 and Its Major Active Metabolite in Human Plasma Using LC-MS/MS
AAPS 2016 -- RG-012 (Parent drug: RG456070) is a single-stranded chemically modified oligonucleotide that binds to and inhibits the activity of miR-21, a microRNA associated with kidney stress and renal dysfunction. Here we report a sensitive, specific, and high throughput LC-MS/MS assay for the accurate quantitation of the parent drug and its major metabolite (RG0005) in human plasma samples with a linear range of 10-5,000 ng/mL
Bioanalysis of Emixustat (ACU-4429) in Whole Blood Collected with Volumetric Absorptive Microsampling by LC-MS/MS
ASMS 2016 -- The analysis of dried-blood spots (DBS) for assessment of PK exposure shows some advantages over traditional plasma-based methods, including simplified collection, processing, storage and shipment. However, bioanalysis of DBS does have challenges related to method validation in accordance with regulatory guidance. Considerations include potential cross contamination during automated excision, sample dilution procedures, application of the internal standard, stability...
Determination of Nutritional and Cyclopropenoid Fatty Acids in Cottonseed by a Single GC Analysis
AOCS 2014 - Historically, a complete analysis of cottonseed fatty acids required two separate procedures: the nutritional fatty acids, as methyl esters, by gas chromatography (GC) and the cyclopropenoid fatty acids (CPEFA), as phenacyl derivatives, by high-pressure liquid chromatography (HPLC). The ring structures of CPEFA interact adversely with packed GC columns, leading to the HPLC methodology, which is labor intensive with long injection times and sub-optimal chromatography.