• Quantitation of Biotherapeutics via LCMS-Surrogate Peptide versus Intact Mass Analysis

    Posters
    Nov. 03, 2019

    AAPS PharmSci 360 2019 -- Therapeutic antibodies have increasingly been utilized for cancer treatment and other therapeutic interventions. Liquid chromatography and tandem mass spectrometry (LC-MS/MS) is used as an alternative platform to ligand binding assays (LBA) as an accurate and reliable technique to measure these proteins in biological fluids. Mass spectrometry-based quantitation of the therapeutic antibodies is generally performed using the surrogate peptide approach, however intact mass analysis is also of interest within the industry. The surrogate peptide approach is known to offer good sensitivity and is amenable to conventional MRM quantitation whereas the intact mass analysis enables the quantitation of protein at an intact level which provides structural information that LBA infers from using specific capture and detection reagents. Within the intact mass analysis approach, variants of data processing are also reported to play a key role in the assay performance.

    AAPS PharmSci 360 2019 -- Therapeutic antibodies have increasingly been utilized for cancer treatment and other therapeutic interventions. Liquid chromatography and tandem mass spectrometry (LC-MS/MS) is used as an alternative platform to ligand binding assays (LBA) as an accurate and reliable technique to measure these proteins in biological fluids. Mass spectrometry-based quantitation of the therapeutic antibodies is generally performed using the surrogate peptide approach, however intact mass analysis is also of interest within the industry. The surrogate peptide approach is known to offer good sensitivity and is amenable to conventional MRM quantitation whereas the intact mass analysis enables the quantitation of protein at an intact level which provides structural information that LBA infers from using specific capture and detection reagents. Within the intact mass analysis approach, variants of data processing are also reported to play a key role in the assay performance.