In Vitro Alternative Test Methods

Chemical Testing

In vitro testing for the assessment of your chemical is increasingly important as animal use continues to be replaced, refined and reduced. Covance is helping lead innovation in this area so by partnering with us you will benefit from extensive experience in standard techniques and novel solutions. Our goal is to provide an approach tailored to address your regulatory needs and aligned to your substance’s specific properties.

In Vitro

Proven expertise in delivering high quality in vitro safety assessments

  • Over 20 years’ experience in designing and conducting a comprehensive suite of in vitro safety assessments to globally recognized standards
  • Innovators in in vitro study design and technology: enhancing study reliability and improving outcomes
  • Insights gathered from worldwide regulatory environments leveraged to smooth the regulatory journey of your chemical 

Your Needs

In vitro safety assessments

An array of in vitro test methods may be employed to determine the potential impact that your chemical has on human safety. With an increased focus on expanding and exploiting in silico and in vitro testing to answer regulatory questions and meet ever-changing endpoints, you need a partner with insight and experience in both in vitro test methods and their applicability to regulatory requirements from around the globe.

Our Capabilities

Covance is at the forefront of in vitro safety assessment   innovation

Covance has played a part in the validation of various in vitro technologies. In addition, staff maintain membership of a range of professional bodies such as the In Vitro Toxicology Society (IVTS), In Vitro Testing Industrial Platform (IVTIP) and the European Society of Toxicology In Vitro (ESTIV) to stay abreast of innovation.

This puts us at the forefront of in vitro methodologies and innovations, generating insights that benefit you as our partner.

Partner with an expert team dedicated to delivering in vitro testing success

Our in vitro testing team consists of experts from diverse backgrounds with extensive experience in planning and managing in vitro studies. The team includes toxicologists, biologists, analytical chemists and environmental scientists. By working collaboratively, study plans can be flexed and optimized.

Let Covance leverage their extensive in vitro testing capabilities to reliably characterize the safety profile of your chemical 

Covance conducts approximately 400 in vitro toxicology studies each year covering the following:


Balb/c 3T3

OECD 432


Balb/c; L929; MRC5


Skin corrosion

3D models

OECD 431


OECD 435

Skin irritation

3D models

OECD 439

Eye irritation


OECD 437

Isolated Chicken Eye Test (with optional histopathology)

OECD 438


OECD 492

Skin sensitization*

Direct Peptide Reactivity Assay (DPRA)


KeratinoSens™ Assay


Human Cell Line Activation Test (hCLAT)


Endocrine Disruption

Estrogen receptor agonist/antagonist

OECD 455

H295R Steriodogenesis assay

OECD 456

BG1Luc estrogen receptor

OECD 458

Human Recombinant Estrogen Receptor (hrER)

OECD 493

*In Vivo Skin Sensitisation (OECD 429) can be offered when justified