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Showing results 945 - 960 of 91813 for .

Showing results 945 - 960 of 91813 for .

Adaptation of the TruCulture(R) System for Flow Cytometry Analysis of Leukocyte Activation Markers
CYTO Congress 2016 -- Evaluation of the immune system is essential in medical research and clinical patient care. Enhancement of immune responses to tumor cells is currently the most promising and active area in cancer drug development. While it is advantageous to assay immune cells as soon as possible after specimen collection, complex laboratory procedures are difficult to implement in multi-site clinical studies.
Basophil Activation Test: Validation of a Flow Cytometry-Based Ex Vivo Pharmacodynamic Marker
CYTO 2017 -- Bruton tyrosine kinase (Btk) activity inhibitors such as PRN1008 and Dasatinib have potent immunosuppressive and anti-inflammatory effects. These properties make them ideal candidates for the therapies of immunologic and inflammatory disorders. Activation of basophils by cross-linking of IgE and FcϵRI is Btk-dependent. Thus, ex vivo basophil activation is a useful PD marker for the dose-dependent mechanism of action of Btk inhibitors. However, validation and implementation of a...
Bringing Basophils Beyond Allergy Testing to Pharmacodynamic Biomarker for Clinical Trials
ICCS 2016 -- Compounds that block Bruton tyrosine kinase (Btk) activity such as Dasatinib and PRN1008 have potent immunosuppressive and anti-inflammatory effects and are being evaluated as therapies for immunologic and inflammatory disorders. Given that IgE -mediated basophil activation via FcϵRI cross-linking is Btk-dependent, ex vivo basophil activation is a useful PD marker for the dose-dependent mechanism of action of these compounds. Due to the low basophil counts in peripheral blood...
Transfer of potency bioassays to a GLP environment, using ADCC reporter bioassays as a case study
AAPS-NBC 2014 - Functional, MOA-based biological assays are required for lot release and stability testing in biologics drug development. Classic assays used to measure the Fc effector function for the therapeutic antibody in antibody-dependent cell-mediated cytotoxicity (ADCC) requires primary effector cells...
The Validation of an Immunoassay for the Determination of Anti-Survivin Antibodies in Human Serum After Survivin Vaccine Administration
AAPS 2014 - Survivin is a member of the inhibitor-of-apoptosis family. Essential for tumor cell survival and overexpressed in most cancers, survivin is a promising target for anti-cancer immunotherapy...
The development and validation of an ultra-sensitive double antigen ELISA method for the determination of bevacizumab (Avastin®) in human serum
AAPS-NBC 2014 - Bevacizumab (Avastin®) is a recombinant human IgG1 monoclonal antibody specific for all human vascular endothelial growth factor-A (VEGF-A) isoforms. In this poster, we report on the development and validation of an ultra-sensitive method for assessment of the presence of this molecule, which may be used to support clinical trials...
The Development and Validation of an Ultra-Sensitive and High-Throughput Fluorometric Method for the Determination of Chitotriosidase in Human Serum
8th Workshop on Recent Issues in BioA (WRIB) 2014 - The underlying defect in Gaucher's disease is an inherited deficiency in enzyme activity of glucocerebrosidase. As a result, the capacity of cells in the body to degrade or breakdown the natural lipid (fatty substance) glucocerebroside is impaired...
The development and validation of an ultra-sensitive ELISA method for the determination of Trastuzumab (Herceptin®) in human serum
AAPS 2104 - HER2 is a member of the epidermal growth factor receptor (EGFR/ERBB) family. The HER2 pathway promotes cell growth and division when it is functioning normally; however, when it is overexpressed, cell growth accelerates beyond its normal limits...
The Comparison of a Mass Spectrometry (MS/MS) and Electrochemiluminescence Immunoassay for the Determination of IgG4 Therapeutic Drugs in Human Serum
AAPS 2014 - In this paper, we report on the use of an immunocapture technique followed by an acid dissociation clean-up procedure and protein digestion with MS detection to allow for a high throughput sample analysis. This approach is then compared to an ELISA method...
The Development of an IA-MS/MS Sensitive and High-Throughput Method for the Determination of IgGx Therapeutic Drugs in Human Serum
8th Workshop on Recent Issues in BioA (WRIB) 2014 - Mass spectrometry is rapidly becoming a fundamental tool for biologists and biochemists in their large molecule therapeutic drug development...
The qualification of a multiplex hybridization-based assay using the xMAP® Luminex® magnetic beads for the determination of mRNA levels in whole blood
Land O' Lakes BioA 2014 - The accurate assessment of mRNA levels in clinical specimens is important for a variety of uses including characterization of disease and therapeutic decision making. In this poster we report on the validation of a multiplex assay with a limit of quantitation of 1 pg/mL for the determination of ten mRNAs in whole blood...
The Validation of a Multiplex Hybridization-Based Assay Using the xMAP® Luminex® Magnetic Beads for the Determination of Ten mRNAs in Human Whole Blood
AAPS 2014 - Messenger RNA (mRNA) is a nontoxic molecule that allows transient protein expression in virtually all cell types and doesn't require nuclear localization or transcription; therefore, it is a very safe biomolecule...
Development of Multi-Class, Multi-Residue UHPLC-MS/MS Method for Screening/Quantification of Veterinary Drugs in Food Matrices and Related Products
ASMS 2016 -- Veterinary drugs are a complex group of different chemical classes and therapeutic agents. They are used within animal husbandry to treat and prevent disease and ensure animal health and growth. Residues of such drugs in animal edible tissues are not desirable because they could pose a potential threat to consumer health and promote antibiotic resistant bacteria strains. Therefore, these substances are strictly regulated and monitored in food products to ensure food safety and...