Immunohistochemistry (IHC) for Oncology

Our preclinical oncology team offers in-house histology and immunohistochemistry (IHC) in addition to other in vitro services for oncology, further expanding our extensive list of services to enhance your study. Optimal preservation and structural integrity of samples is ensured by simply moving samples down the hallway to the histology lab in our state-of-the-art facility. 

Our high-quality IHC assays give you a time-efficient read-out on quantitative and qualitative data and can be produced expeditiously that reveal the following:

  • Efficacy of your compound in target tissues
  • Location of biomarkers in tissues
  • Incidence of tumor metastasis
  • Occurrence of tissue transformation into tumors
  • Origin of tumor tissue
  • Prognosis of the animals in the study

Featured IHC Articles:

 

Services:

  • Normal Tissue
  • Tumor Tissue
  • Xenograft
  • New Marker Validation
  • Immunofluorescence
  • Immunocytochemistry
  • Multiplex – Custom markers with up to four-color immunofluorescence

 

Validated IHC Markers:

AE1/AE3+5D3
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AE1/AE3+5D3 cytoplasmic positive mouse skin. Cytokeratin’s are  filamentous proteins detectable in epithelial cells. They can be utilized in tumor differentiation, cell typing, and micrometastasis.

CD3
CD3 membrane staining in spleen

CD3 membrane staining in spleen

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CD3 staining in 4T1

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CD3 staining in A20

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CD3 staining in CT26

Mouse CD3 membrane staining in spleen. CD3 is used to identify T cells in normal tissue, as well as T cell neoplasms.

CD4
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CD4 staining in subcutaneous A20

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CD4 immunofluorescent staining in A20

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CD4 staining in CT26

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CD4 staining in Pan02

Mouse CD4 membrane staining in mouse tumor tissue. CD4 is used to identify T helper cells, and plays a role in T cell activation.

CD8
Mouse CD8 membrane

Mouse CD8 membrane staining in subcutaneous CT26 mouse tumor tissue. CD8 is used to identify cytotoxic T cells.

CD19
CD19 membrane

Human CD19 membrane staining in human tonsil. CD19 is a biomarker for B cells, and plays a role in B cell development.

CD20
CD20 staining

Human CD20 staining in human tonsil. CD20 is a B cell marker whose function is to enable optimal B-cell immune response.

CD22
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Human CD22 staining in human tonsil. CD22 is found on the surface of mature B cells that functions as an inhibitory receptor for B cell receptor signaling. It is an active target for B cell malignancies and autoimmune disease.

CD38
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CD38 staining in 4T1

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CD38 staining in A20

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CD38 staining in CT26

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CD38 staining in ID8

Mouse CD38 staining in multiple tissues, as indicated. CD38 is found on the surface of many immune cells (CD4+ and CD8+ T cells, B cells, and natural killer cells). It has functions in cell adhesion, signal transduction and calcium signaling and is used as a prognostic marker in leukemia patients with CLL.

CD45
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Mouse CD45 membrane staining in A20 tumor tissue. CD45 plays a role in the regulation of T- and B-cell antigen receptor signaling.

CD163
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CD163 staining in CT26

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Mouse CD163 staining in multiple tissues, as indicated. CD163 is expressed in macrophages and monocytes. Expression may be indicative of inflammation.

FoxP3
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Mouse FOXP3 nuclear staining in spleen. FoxP3 is involved in the development and inhibitory function of regulatory T cells.

HER2
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Her2 positive cytoplasm in BT474 tumor tissue.

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HER2 Immunofluorescence of SK-OV-3 Cells

 

Her2 is a human EGF receptor involved in kinase mediated activation of signaling pathways. It is used as a predictive factor and determinant in therapy in cancer treatment.

Ki67
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Human Ki-67 nuclear staining in human tonsil. The Ki-67 protein is associated with cellular proliferation, and can be used as a prognostic tool in cancer.

PR
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Human PR positive nuclei in BT474 tumor tissue. Progesterone receptor is involved in activating signaling pathway for hormone stimulation. It can be utilized in determining appropriate drug  therapy in cancer treatment.