Cell Culture

Large-scale culture
file
file

Covance can grow and expand your cell lines for implantation into hundreds of mice or freeze as many vials as necessary at the cell concentration you need. We have fourteen CO2 incubators, three full-sized biosafety cabinets, two cell automated cell counters, and a vast amount of experience growing hundreds of different cell lines in dishes, flasks, roller bottles, spinner flasks, and multi-layered flasks.

Cell morphology
file

Covance can monitor the cell morphology of your cells in culture using an Olympus CKX53F inverted fluorescence microscope with phase contrast and a 1.4MP CCD camera. View pictures of your cells before, during, and after treatment, over extended periods of time, or at specific time points before implantation to verify that the cells are growing normally.

Cell growth/tumorigenicity
file

 

We can monitor the growth of your cells over time and compare different cell variants, cells grown in different media, or cells grown with different treatments. We have two Luna Automated Cell Counters that can accurately distinguish the live from the dead cells and count each population. Our cell culture team has a vast amount of experience culturing multiple types of cells including tumor cells, primary cells, and neurospheres. The team also has experience performing colony formation assays to detect the tumorigenic potential of transformed cells and the tumor suppressive effects of proteins on transformed cells in vitro. Our flow cytometry team has experience monitoring the proliferation of stimulated T-cells or T-cells in mixed lymphocyte reactions using CFSE staining.

High-content image analysis
file
file

Covance has the ability to stain cells and acquire fluorescent images using the Cytation 3 Imaging Plate Reader. Once the images have been captured, we have specialized software that can count nuclei, count live vs dead cells, count stained cells as positive vs. negative, and measure the size, circularity, area, perimeter, and intensity of each color in the images.

Image-enabled cell line generation
file
file

Firefly (P. pyralis) luciferase is an enzyme that produces bioluminescence in the presence of D-luciferin. The DNA for the luciferase 2 (luc2) gene, along with a puromycin resistance gene, is incorporated into the genome of the cells using a lentiviral transduction system. While culturing the cells, puromycin is added to the medium to select only the cells with the inserted luciferase gene. Once the luc-enabled cells are injected into a mouse or rat and D-luciferin is administered, the light produced can be detected with Covance's IVIS® Spectrum in vivo imaging system. The in vitro services team has experience performing every step of the process from stably transducing cells, to selecting the transduced cells, to measuring the light output of the cells.

Read our related blog article regarding luciferase

 
Cytotoxicity / proliferation assay
file

Cytotoxicity/proliferation assays are widely used to screen for cytotoxicity in compound libraries. If you are interested in developing a therapeutic agent that targets rapidly dividing cancer cells, we can test your cytotoxic compounds. We can also screen “hits” from initial high-throughput drug screens for unwanted cytotoxic effects before you invest in your agent as a pharmaceutical. Covance can measure the toxicity of your compound in your choice of over 400 cell lines that we have available. We provide graphs and IC50 values for each cell line and compound.