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Small interfering RNA (siRNA) quantitation
Synthetic small interfering RNAs (siRNAs) can be introduced into a diverse range of organisms and cell types to induce degradation of MicroRNAs (mRNAs) with sequence complementarity, inducing short-term silencing of protein coding genes. RNA Interference (RNAi) has been used for applications that include gene function and pathway analysis, target identification and validation, and therapeutics. Quantitation of siRNAs in cells and animal tissues provides essential information on siRNA delivery efficiency, distribution, stability and specificity.
