Covance Genomics Laboratory has developed solutions for efficient analysis of next generation sequence data. Our sequencing services offer advantages in time, cost, and sensitivity. Using Illumina’s and Life Technologies industry-leading sequencing methods, CGL generates high-throughput, powerful data for your research.

Our comprehensive sequencing services are thoughtfully designed to seemlessly handle your project from sample to data. In addition, our partnership with Ingenuity and Illumina allows us to analyze and produce high-quality data in a fraction of the time than our competitors offer.

Our Sequencing Data Quality
Covance Genomics Laboratory has performed extensive in-house testing to measure our data quality and specificity. Our information rich data identifies genomic aberrations and is highly reproducible. We offer 35 to 100 base pair reads (single or paired end) with greater than 85% high quality bases (Q>30).

Applications

• miRNA sequencing (TruSeq Small RNA)
• Whole transcriptome sequencing
• Targeted Resquencing, PCR/Capture
• ChiP Seq, RIP-Seq
• Methylation
• Targeted Selection Genotyping
• Whole Genome/Exome
• NuGEN low input amplification is used for precious samples

Platforms

Illumina HiSeq 2000G
With up to 2 x 100 bp runs, HiSeq 2000 platform is ideal for:

• Whole Genome Re-sequencing
• Targeted Resequencing
• De Novo Sequencing
• Transcriptome Sequencing

Illumina MiSeq

• Highly Multiplexed PCR
• Amplicon Sequencing
• Targeted Resequencing
• De Novo Sequencing
• Small RNA Sequencing
• 16S Metagenomics RNA-Seq

Ion Torrent PGM
This high speed system offers 1 x 200 base reads in two hours. Applications include but are not limited to:

• Amplicon Sequencing
• Microbial Sequencing
• RNA Sequencing (RNA-Seq)
• Targeted Sequencing
• Mitochondrial Sequencing
• Methylation Analysis

Fluidigm

• Used for sample enrichment

Data Analysis with Ingenuity iReport
To offer our customers the latest technology advances, we use several open source tools like bwa, bowtie, tophat, samtools, GATK, etc. In addition, we have collaborated with Ingenuity, running their analytic tools for further downstream analysis of Next Generation sequencing data. By employing Ingenuity's extensively curated scientific literature, clients will be able to mine complex next generation sequence data as well as extract actionable and meaningful information in a fraction of time.

The Covance Difference
CGL has extensive experience in developing and validating CLIA assays for mutational analysis. Our assays are highly reproducible and sensitive to detect mutations.

Sequencing of specific regions of interests by NGS also allows identification of novel single nucleotide variants and their allele frequencies in the target region(s).

Scientists at CGL work with clients to develop novel assays to facilitate the scientific discoveries.