The Covance Genomics Laboratory specializes in high quality, high throughput gene expression profiling. We offer end-to-end services from sample preparation to data analysis, all tracked within our LIMS system. CGL's portfolio of capabilities covers the spectrum from low complexity assays to whole genome profiling. By identifying genes that are differentially expressed in particular diseases or patient populations, new targets can be identified and validated for the development of new therapies. Gene expression analysis may also be leveraged for determining clinical trial inclusion and exclusion criteria.
Get in touch with our Client Services Team to determine which platform best meets your project’s needs.
The Covance Genomics Laboratory employs the NanoString Gen2 nCounter platform to analyze anywhere from 20-800 genes. This system has several benefits, including the ability to measure gene expression directly from lysates—no RNA preparation needed.
As a highly reproducible and sensitive platform, it is valuable for gene expression profiling in various sample types including fresh frozen and FFPET samples, as demonstrated in our recent publication, Multiplexed measurements of gene signatures in different analytes using the Nanostring nCounter™ Assay System.
»Read the publication here
The nCounter System utilizes non-enzymatic detection, which reduces the susceptibility to inhibitors found in certain sample types, like FFPE. With our purpose-built space, CGL is NanoString’s only commercial service provider for the nCounter Analysis System.
»Learn more about nCounter platform on the NanoString website
As an Affymetrix Authorized Service Provider, Covance Genomics Laboratory offers an extensive collection of Affymetrix GeneChip analysis tools. CGL offers both custom and catalog arrays. The Affymetrix catalog whole genome arrays include human, rat and mouse (Human Genome U133 Plus 2.0 Array, the Mouse Genome 430A 2.0 Array, and the Rat Genome 230 2.0 Array). CGL also offers custom whole genome arrays for human, rat, and mouse designed by key experts from Rosetta Inpharmatics and Merck. Hybridization to other Affymetrix catalog arrays is also available; email our Client Services Team for information.
CGL provides gene expression profiling with Agilent's 60-mer oligonucleotide microarrays. This platform was originally developed by Rosetta Inpharmatics LLC and licensed to Agilent Technologies, Inc. The platform is highly flexible with easily revisable pattern files. CGL offers many different array patterns for various organisms and has experience in the design and validation of new patterns. This platform is also used for miRNA profiling.
Next Generation Sequencing:
Illumina HiSeq, MiSeq, and Life Technologies Ion Torrent PGM
Our Next Generation Sequencing technology can be used for both gene expression and the identification of expressed SNVs. Read more about our developed solutions for efficient analysis of next generation sequence data on the Next Generation Sequencing page.
Real-Time Quantitative PCR – Applied Biosystems TaqMan® Gene Expression Assays
As the "gold standard" for gene expression, Covance Genomics Laboratory offers TaqMan for targeted gene expression of small panels of genes, from a single gene up to 384.
TaqMan Array Cards (TAC) formats are also available. This microfluidic card is ideal for gene panels that will be repeatably used in multiple studies. With high specificity, sensitivity, and broad dynamic range, the TAC platform can be a powerful and cost-effective service for certain types of projects.
Related Gene Expression Services:
- Custom Assay Design and Biomarker Validation
Gene Expression can also be used for target engagement and measuring pharmacodynamic response. Along with these biological measures, CGL offers biological validation tailored directly to a study's objectives and development stage. Read more about this topic on our Translational Biomarkers page.
- Custom Amplification Techniques
Using both Agilent and Affymetrix platforms, we offer multiple protocols for amplifying RNA from difficult, sample-limited, or degraded samples with as little as 1 ng total RNA.
Read an example of this in Neuron: Transcriptional Architecture of the Primate Neocortex
Volume 73, Issue 6, 1083-1099, 22 March 2012.