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Custom Immunology Services
Sample Polyclonal Schedules of Events |
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| Rat Protein Schedule of Events, 115 Day |
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| Day |
Procedure |
| 0 |
Rat
Pre-bleed (Avg. 0.5 mL serum)
1° SC: 200 µg with FCA |
| 21 |
Boost SC: 100 µg with FIA |
| 31 |
Test Bleed (Avg. 0.5 mL serum) |
| 42 |
Boost SC: 100 µg with FIA |
| 52 |
Production Bleed (Avg. 2 mL serum) |
| 53 |
ELISA Titer Assay of Bleed* |
| 63 |
Boost SC: 100 µg with FIA |
| 73 |
Production Bleed (Avg. 2 mL serum) |
| 84 |
Boost SC: 50 µg with FIA |
| 94 |
Production Bleed (Avg. 2 mL serum) |
| 95 |
ELISA Titer Assay of Bleed* |
| 105 |
Boost SC: 50 µg with FIA |
| 115 |
Project Completion** |
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| **contact us for further details regarding project completion |
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Total material required for injection for the above program is 600 µg/animal. If we are conjugating your protein we assume a 20% loss during conjugation. Total amount of serum expected from a rat on the above program is approximately 13.5 mL.
It is recommended that a minimum of three rats be used due to biological variations.
Immunogen is emulsified in Freund's Complete Adjuvant (FCA) for initial injections. Freund's Incomplete Adjuvant (FIA) is used for all subsequent injections (boosts). Projects follow a three-week cycle of boosts. Test bleeds are taken approximately 10 days after the boosts.
*Optional ELISA: We suggest testing the first bleed of the project to evaluate the effect of antigen on the animal. Information gained from an ELISA at this point will provide an opportunity for you to make adjustments to your immunization schedule. Additional ELISA's performed later in the project will give you valuable information that can be used to determine which sera samples to include in your research or which bleeds you would like to have purified.
Download needed order forms. We'll process your order immediately.
Our Shipping Address
Attn: Immunology Services
465 Swampbridge Road
Denver, PA 17517
tel (800) 345-4114
tel (717) 336-4921
fax (717) 336-3481
AAALAC International Accredited, GLP Studies, OLAW Assured, USDA Research Registered
Document Revision Date: 6/12/07 |
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