Home About Covance Investor Relations News Careers Contact Us Home > Drug Development Services > Nonclinical > Antibody Services > Polyclonal Production  Advanced Search Antibody Services Overview Polyclonal Production   Sample Schedules of Events   Technical Tips Hybridoma Development Monoclonal Production Immunochemistry Services News, Events & Publications Industry Links Quote Request Custom Immunology Services Sample Polyclonal Schedules of Events Guinea Pig Protein Schedule of Events, 115 Day Day Procedure 0 Guinea Pig Pre-bleed (Avg. 1 mL serum) 1° SC: 200 µg with FCA 21 Boost SC: 100 µg with FIA 31 Test Bleed (Avg. 1 mL serum) 42 Boost SC: 100 µg with FIA 52 Production Bleed (Avg. 2 mL serum) 53 ELISA Titer Assay of Bleed* 63 Boost SC: 100 µg with FIA 73 Production Bleed (Avg. 2 mL serum) 84 Boost SC: 100 µg with FIA 94 Production Bleed (Avg. 2 mL serum) 95 ELISA Titer Assay of Bleed* 105 Boost SC: 100 µg with FIA 115 Project Completion** **contact us for further details regarding project completion Total material required for injection for the above program is 700 µg/animal. If we are conjugating your protein we assume a 20% loss of protein during conjugation. Total amount of serum expected from a guinea pig on the above program is approximately 17.0 mL. It is recommended that a minimum of three guinea pigs be used due to biological variations. Immunogen is emulsified in Freund's Complete Adjuvant (FCA) for initial injections. Freund's Incomplete Adjuvant (FIA) is used for all subsequent injections (boosts). Projects follow a three-week cycle of boosts. Test bleeds are taken approximately 10 days after the boosts. *Optional ELISA: We suggest testing the first bleed of the project to evaluate the effect of antigen on the animal. Information gained from an ELISA at this point will provide an opportunity for you to make adjustments to your immunization schedule. Additional ELISA's performed later in the project will give you valuable information that can be used to determine which sera samples to include in your research or which bleeds you would like to have purified. Download needed order forms. We'll process your order immediately. Our Shipping Address Attn: Immunology Services 465 Swampbridge Road Denver, PA 17517 tel (800) 345-4114 tel (717) 336-4921 fax (717) 336-3481 AAALAC International Accredited, GLP Studies, OLAW Assured, USDA Research Registered Document Revision Date: 6/12/07 Antibody Services Tel: 800-345-4114 Tel: 717-336-4921 Fax:717-336-3481 Contact Us Quote Request News